Molecular-genetic identification of microorganism strains isolated from activated sludge based on an analysis of nucleotide sequences of the 16s rRNA gene

A molecular-genetic identification of four bacterial strains isolated from activated sludge urban wastewater treatment plants (Ulan-Ude) and the industrial enterprise OJSC “Selenginsky Pulp Paper Mill” (Selenginsk) was carried out. Bacterial were identified by a capillary sequencer ABI 3130XL Genetic Analyzer (Applied Biosystems) using 16S primers 27F 1492R at Genomics Collective Use Center Institute Chemical Biology Fundamental Medicine, SB RAS, Novosibirsk. The results obtained method determination direct nucleotide sequence rRNA fragment followed comparison identity with sequences deposited in international database GenBank. according to GenBank database: strain B 1.1 corresponds Paenibacillus dendritiformis P411 (similarity 99.93%), 1.2 1.3 correspond Bacillus licheniformis PB399 86 100%, respectively), P polymyxa ISSDS-85 99.86%). biochemical properties determined: amylolytic, proteolytic lipolytic activity; ability ferment carbohydrates Hiss’ nutrient medium; form ammonia, urea nitrate reduction. may be promising for destruction pollutants. On their basis, it is planned create consortium microorganisms protein fatty pollutants wastewater.